Difference between revisions of "Part:BBa K2984009:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Codon optimization
+
Codon syntax fits <i>C. reinhardtii</i>. This was designed as a A1-A3 part according to the MoClo standard of Patron et al. (2015).
  
  
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
(Schroda et al. 2000)
+
Schroda, M., et al. (2002). "Sequence elements within an HSP70 promoter counteract transcriptional transgene silencing in Chlamydomonas." Plant J 31(4): 445-455.
  
 
===References===
 
===References===
 +
 +
Schroda, M., et al. (2002). "Sequence elements within an HSP70 promoter counteract transcriptional transgene silencing in Chlamydomonas." Plant J 31(4): 445-455.
 +
Patron, N. J., Orzaez, D., Marillonnet, S., Warzecha, H., Matthewman, C., Youles, M., . . . Haseloff, J. (2015). Standards for plant synthetic biology: a common syntax for exchange of DNA parts. New Phytologist, 208(1), 13-19. Retrieved from https://nph.onlinelibrary.wiley.com/doi/abs/10.1111/nph.13532.
 +
Schroda, M., et al. (2000). "The HSP70A promoter as a tool for the improved expression of transgenes in Chlamydomonas." Plant J 21(2): 121-131.

Latest revision as of 18:53, 15 October 2019


AR Promoter A1-A3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 282
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 685
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon syntax fits C. reinhardtii. This was designed as a A1-A3 part according to the MoClo standard of Patron et al. (2015).


Source

Schroda, M., et al. (2002). "Sequence elements within an HSP70 promoter counteract transcriptional transgene silencing in Chlamydomonas." Plant J 31(4): 445-455.

References

Schroda, M., et al. (2002). "Sequence elements within an HSP70 promoter counteract transcriptional transgene silencing in Chlamydomonas." Plant J 31(4): 445-455. Patron, N. J., Orzaez, D., Marillonnet, S., Warzecha, H., Matthewman, C., Youles, M., . . . Haseloff, J. (2015). Standards for plant synthetic biology: a common syntax for exchange of DNA parts. New Phytologist, 208(1), 13-19. Retrieved from https://nph.onlinelibrary.wiley.com/doi/abs/10.1111/nph.13532. Schroda, M., et al. (2000). "The HSP70A promoter as a tool for the improved expression of transgenes in Chlamydomonas." Plant J 21(2): 121-131.