Difference between revisions of "Part:BBa K2965031"

Latest revision as of 17:21, 15 October 2019

Lba-crRNA-HPV16-1

This part is a crRNA of LbaCas12a, it is complementary to HPV16 L1 sequence. There is a 5' TTTN PAM on the DNA strand opposite the target sequence. When LbaCas12a is guided by this crRNA, once it recognize its target, it will be activated and exhibits a “collateral effects” of promiscuous DNase activity.

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 16:57, 15 October 2019 (view source) LIWENXIAO (Talk \| contribs) (→‎Usage and Biology) ← Older edit		Latest revision as of 17:21, 15 October 2019 (view source) LIWENXIAO (Talk \| contribs)
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	<partinfo>BBa_K2965031 short</partinfo>		<partinfo>BBa_K2965031 short</partinfo>

−	This part is a crRNA of LbaCas12a ~~which~~ is complementary to HPV16 L1 sequence. There is a a 5' TTTN PAM on the DNA strand opposite the target sequence. When LbaCas12a is guided by this crRNA, once it recognize its target, it will be activated and exhibits a “collateral effects” of promiscuous DNase activity.	+	This part is a crRNA of LbaCas12a, it is complementary to HPV16 L1 sequence. There is a 5' TTTN PAM on the DNA strand opposite the target sequence. When LbaCas12a is guided by this crRNA, once it recognize its target, it will be activated and exhibits a “collateral effects” of promiscuous DNase activity.