Difference between revisions of "Part:BBa K2965036"

 
Line 3: Line 3:
 
<partinfo>BBa_K2965036 short</partinfo>
 
<partinfo>BBa_K2965036 short</partinfo>
  
−
CC
+
This part is a crRNA of AsCas12a which is complementary to HPV16 L1 sequence. There is a a 5' TTTN PAM on the DNA strand opposite the target sequence. When LbaCas12a is guided by this crRNA, once it recognize its target, it will be activated and exhibits a “collateral effects” of promiscuous DNase activity.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:11, 15 October 2019


As-crRNA-HPV16-3

This part is a crRNA of AsCas12a which is complementary to HPV16 L1 sequence. There is a a 5' TTTN PAM on the DNA strand opposite the target sequence. When LbaCas12a is guided by this crRNA, once it recognize its target, it will be activated and exhibits a “collateral effects” of promiscuous DNase activity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]