Difference between revisions of "Part:BBa K2965031"
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This part is a crRNA of LbaCas12a which is complementary to HPV16 L1 sequence. There is a a 5' TTTN PAM on the DNA strand opposite the target sequence. When LbaCas12a is guided by this crRNA, once it recognize its target, it will be activated and exhibits a “collateral effects” of promiscuous DNase activity. | This part is a crRNA of LbaCas12a which is complementary to HPV16 L1 sequence. There is a a 5' TTTN PAM on the DNA strand opposite the target sequence. When LbaCas12a is guided by this crRNA, once it recognize its target, it will be activated and exhibits a “collateral effects” of promiscuous DNase activity. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K2965031 parameters</partinfo> | <partinfo>BBa_K2965031 parameters</partinfo> | ||
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Revision as of 16:54, 15 October 2019
Lba-crRNA-HPV16-1
This part is a crRNA of LbaCas12a which is complementary to HPV16 L1 sequence. There is a a 5' TTTN PAM on the DNA strand opposite the target sequence. When LbaCas12a is guided by this crRNA, once it recognize its target, it will be activated and exhibits a “collateral effects” of promiscuous DNase activity.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]