Difference between revisions of "Part:BBa K2912016"

Revision as of 15:59, 15 October 2019

G-quadruplexes used to detect miRNA

MicroRNAs (miRNAs) have been considered to be potent biomarkers for early disease diagnosis and for cancer therapy. The rapid and selective detection of miRNAs without reverse transcription and labelling is highly desired. Herein, we reported a simple and label-free miRNA detection method.The interaction of the G-quadruplex DNA structure with Thioflavin T (ThT) led to a label-free signal output. Under the optimised conditions, this method allowed for simple, rapid, and sequence-specific detection of miRNA. SZU-China this year used G-quadruplexes to detect siRNA introduced into the leaves of M. micrantha quantatively.

Sequence and Features