Difference between revisions of "Part:BBa K3096025"

Latest revision as of 15:21, 15 October 2019

pMnt-RFP reporter for Mnt expression

Used to measure the expression of MntR and give a mRFP read out. If pMnt is present, the system should be repressed and no mRFP signal should be detectable.

Usage and Biology

The pMnt is a promoter originating from the Salmonella bacteriophage P22.

This promoter is regualted by MntR. If MntR binds to the promoter transcription of downstream genes is inhibited.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NotI site found at 781
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1555
Illegal AgeI site found at 1667
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
-__NOTOC__
 <partinfo>BBa_K3096025 short</partinfo>
-Used to measure the expression of MntR, thus the sensing of GlcNAc. If GlcNAc is sensed, the reporter should lose its ability to express RFP.
+Used to measure the expression of MntR and give a mRFP read out. If pMnt is present, the system should be repressed and no mRFP signal should be detectable.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+The pMnt is a promoter originating from the Salmonella bacteriophage P22.
+This promoter is regualted by MntR. If MntR binds to the promoter transcription of downstream genes is inhibited.
-<!-- -->
+===Sequence and Features===
-<span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K3096025 SequenceAndFeatures</partinfo>
+==References==
+#
 <!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
 <partinfo>BBa_K3096025 parameters</partinfo>
 <!-- -->