Difference between revisions of "Part:BBa K3096018"

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N-Acetyl Glucosamine is released from commensal bacteria in the human microbiome metabolising the mucus layer of the intestines (Sicard et al. 2017). GlcNAc is taken up by the bacteria through their PTS system and metabolized into GlcNAc-6-P, which binds the repressor protein nagC. If nagC is bound to GlcNAc-6-P it loses its abilities to bind DNA, and therefore its respective regulation activity. In our case, nagC can consequently not serve as a repressor of the nag Operon nagBACDE, anymore (Barhart et al. 2006, Konopka et al 2012).  
 
N-Acetyl Glucosamine is released from commensal bacteria in the human microbiome metabolising the mucus layer of the intestines (Sicard et al. 2017). GlcNAc is taken up by the bacteria through their PTS system and metabolized into GlcNAc-6-P, which binds the repressor protein nagC. If nagC is bound to GlcNAc-6-P it loses its abilities to bind DNA, and therefore its respective regulation activity. In our case, nagC can consequently not serve as a repressor of the nag Operon nagBACDE, anymore (Barhart et al. 2006, Konopka et al 2012).  
  
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===Sequence and Features===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3096018 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3096018 SequenceAndFeatures</partinfo>
  
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===References===
 
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Sicard JF, Le Bihan G, Vogeleer P, Jacques M, Harel J. Interactions of Intestinal Bacteria with Components of the Intestinal Mucus. Front Cell Infect Microbiol. (2017);7:387. Published 2017 Sep 5. doi:10.3389/fcimb.2017.00387
 
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Barnhart MM, Lynem J, Chapman MR. GlcNAc-6P levels modulate the expression of Curli fibers by Escherichia coli. J Bacteriol. (2006);188(14):5212–5219. doi:10.1128/JB.00234-06
 
  
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Konopka JB. N-acetylglucosamine (GlcNAc) functions in cell signaling. Scientifica (Cairo). (2012);2012:489208. doi:10.6064/2012/489208
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==References==
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#'''Sicard JF, Le Bihan G, Vogeleer P, Jacques M, Harel J'''. Interactions of Intestinal Bacteria with Components of the Intestinal Mucus. ''Front Cell Infect Microbiol.'' ('''2017''');7:387. Published 2017 Sep 5. doi:10.3389/fcimb.2017.00387
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#'''Barnhart MM, Lynem J, Chapman MR'''. GlcNAc-6P levels modulate the expression of Curli fibers by Escherichia coli. ''J Bacteriol''. ('''2006''');188(14):5212–5219. doi:10.1128/JB.00234-06
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#'''Konopka JB'''. N-acetylglucosamine (GlcNAc) functions in cell signaling. ''Scientifica (Cairo)''. ('''2012''');2012:489208. doi:10.6064/2012/489208
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
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Latest revision as of 15:19, 15 October 2019

NagC repressor

NagC is a repressor for the nag-operon which is needed for GlcNAc6P catabolism. If nagC is bound by GlcNAc6P it looses its repression capability. NagC is an activator for the glm-operon which is responsible for GlcNAc6P anabolism.

Background

In the project of iGEM Team Tübingen 2019, the NagC repressor was used to sense GlcNAc6P in order to evaluate whether the bacterium is located in permissible conditions.

Usage and Biology

N-Acetyl Glucosamine is released from commensal bacteria in the human microbiome metabolising the mucus layer of the intestines (Sicard et al. 2017). GlcNAc is taken up by the bacteria through their PTS system and metabolized into GlcNAc-6-P, which binds the repressor protein nagC. If nagC is bound to GlcNAc-6-P it loses its abilities to bind DNA, and therefore its respective regulation activity. In our case, nagC can consequently not serve as a repressor of the nag Operon nagBACDE, anymore (Barhart et al. 2006, Konopka et al 2012).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. Sicard JF, Le Bihan G, Vogeleer P, Jacques M, Harel J. Interactions of Intestinal Bacteria with Components of the Intestinal Mucus. Front Cell Infect Microbiol. (2017);7:387. Published 2017 Sep 5. doi:10.3389/fcimb.2017.00387
  2. Barnhart MM, Lynem J, Chapman MR. GlcNAc-6P levels modulate the expression of Curli fibers by Escherichia coli. J Bacteriol. (2006);188(14):5212–5219. doi:10.1128/JB.00234-06
  3. Konopka JB. N-acetylglucosamine (GlcNAc) functions in cell signaling. Scientifica (Cairo). (2012);2012:489208. doi:10.6064/2012/489208