Difference between revisions of "Part:BBa K3241000:Design"

(→‎Source)
(→‎Source)
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This gene is originally from Ralstonia eutropha.GenScript synthesized the gene and optimized its codon for us.
 
This gene is originally from Ralstonia eutropha.GenScript synthesized the gene and optimized its codon for us.
 +
This gene was cloned in pUC57.
  
 
===References===
 
===References===

Revision as of 15:05, 15 October 2019


PhbA(Beta-ketothiolase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1126
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 225
    Illegal NgoMIV site found at 310
    Illegal NgoMIV site found at 715
    Illegal NgoMIV site found at 844
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 141


Design Notes

atgaccgatgttgtcattgtctctgccgctcgtaccgccgttggtaaatttggtggcagcttggccaaaattccggctcctgaattgggcgccgttgtcattaaagccgctttggaacgtgctggtgttaaaccggaacaggtctctgaagttattatgggccaagtcttgaccgctggtagcggtcagaatcctgctcgtcaagccgctattaaagccggtttgccggctatggtccctgccatgaccattaataaagtttgtggctctggtttgaaagccgtcatgttggccgctaatgctattatggccggcgatgctgaaattgttgtcgctggtggccaggaaaatatgtctgccgctccgcatgttttgcctggcagccgtgatggttttcgcatgggcgatgccaaattggtcgataccatgattgttgatggtttgtgggatgtttataatcagtatcatatgggcatcaccgctgaaaatgtcgccaaagaatatggtattacccgcgaagcccaagatgaatttgctgttggtagccagaataaagccgaagccgctcaaaaagctggcaaatttgatgaagaaattgtcccggttttgattcctcagcgtaaaggtgatccggttgcctttaaaaccgatgaatttgtccgccaaggtgctaccttggattctatgagcggcttgaaacctgcttttgataaagccggcaccgttaccgccgctaatgcctctggtttgaatgatggcgccgctgccgttgtcgttatgagcgctgccaaagccaaagaattgggtttgaccccgttggctaccattaaatcttatgctaatgccggcgtcgatcctaaagttatgggcatgggtccggttcctgcttctaaacgtgccttgagccgcgctgaatggaccccgcaggatttggatttgatggaaattaatgaagcctttgctgcccaagctttggccgtccatcagcaaatgggttgggatacctctaaagtcaatgttaatggtggcgctattgccattggccatcctattggcgccagcggttgtcgtattttggttaccttgttgcatgaaatgaaacgtcgcgatgccaaaaaaggcttggctagcttgtgcattggtggcggtatgggtgtcgctttggccgttgaacgcaaataa


Source

This gene is originally from Ralstonia eutropha.GenScript synthesized the gene and optimized its codon for us. This gene was cloned in pUC57.

References