Difference between revisions of "Part:BBa K3096007"
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This is a part consisting of all cofactors necessary for the functionality of the CRISPR/Cas3 system. It works if utilised with BBa_K3096001 and the respective CRISPR arrays targeting the to-be-degraded sequences. | This is a part consisting of all cofactors necessary for the functionality of the CRISPR/Cas3 system. It works if utilised with BBa_K3096001 and the respective CRISPR arrays targeting the to-be-degraded sequences. | ||
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In the type I CISPR/Cas system the crRNA guides the surveillance complex Cascade ABCDE to | In the type I CISPR/Cas system the crRNA guides the surveillance complex Cascade ABCDE to | ||
complementary DNA sequence. The DNA is unwound, generating the so-called R-loop. The Cas3 helicase-nuclease protein attaches to this R-loop and unidirectionally degrades the DNA. Hereby, the large Cas3/Cascade complex pulls the substrate DNA towards itself, introducing single strand breaks. | complementary DNA sequence. The DNA is unwound, generating the so-called R-loop. The Cas3 helicase-nuclease protein attaches to this R-loop and unidirectionally degrades the DNA. Hereby, the large Cas3/Cascade complex pulls the substrate DNA towards itself, introducing single strand breaks. | ||
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| + | ===Sequence and Features=== | ||
| + | <partinfo>BBa_K3096010 SequenceAndFeatures</partinfo> | ||
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| + | ==References== | ||
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Revision as of 15:04, 15 October 2019
Type I CRISPR/Cas system surveillance complex Cascade ABCDE
This is a part consisting of all cofactors necessary for the functionality of the CRISPR/Cas3 system. It works if utilised with BBa_K3096001 and the respective CRISPR arrays targeting the to-be-degraded sequences.
Usage & Biology
In the type I CISPR/Cas system the crRNA guides the surveillance complex Cascade ABCDE to complementary DNA sequence. The DNA is unwound, generating the so-called R-loop. The Cas3 helicase-nuclease protein attaches to this R-loop and unidirectionally degrades the DNA. Hereby, the large Cas3/Cascade complex pulls the substrate DNA towards itself, introducing single strand breaks.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]