Difference between revisions of "Part:BBa K3241000:Design"
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===Source=== | ===Source=== | ||
| − | This gene is from Ralstonia eutropha. | + | This gene is originally from Ralstonia eutropha.GenScript synthesized the gene and optimized its codon for us. |
| − | + | ||
===References=== | ===References=== | ||
Revision as of 15:02, 15 October 2019
PhbA(Beta-ketothiolase)
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1126
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 225
Illegal NgoMIV site found at 310
Illegal NgoMIV site found at 715
Illegal NgoMIV site found at 844 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 141
Design Notes
atgaccgatgttgtcattgtctctgccgctcgtaccgccgttggtaaatttggtggcagcttggccaaaattccggctcctgaattgggcgccgttgtcattaaagccgctttggaacgtgctggtgttaaaccggaacaggtctctgaagttattatgggccaagtcttgaccgctggtagcggtcagaatcctgctcgtcaagccgctattaaagccggtttgccggctatggtccctgccatgaccattaataaagtttgtggctctggtttgaaagccgtcatgttggccgctaatgctattatggccggcgatgctgaaattgttgtcgctggtggccaggaaaatatgtctgccgctccgcatgttttgcctggcagccgtgatggttttcgcatgggcgatgccaaattggtcgataccatgattgttgatggtttgtgggatgtttataatcagtatcatatgggcatcaccgctgaaaatgtcgccaaagaatatggtattacccgcgaagcccaagatgaatttgctgttggtagccagaataaagccgaagccgctcaaaaagctggcaaatttgatgaagaaattgtcccggttttgattcctcagcgtaaaggtgatccggttgcctttaaaaccgatgaatttgtccgccaaggtgctaccttggattctatgagcggcttgaaacctgcttttgataaagccggcaccgttaccgccgctaatgcctctggtttgaatgatggcgccgctgccgttgtcgttatgagcgctgccaaagccaaagaattgggtttgaccccgttggctaccattaaatcttatgctaatgccggcgtcgatcctaaagttatgggcatgggtccggttcctgcttctaaacgtgccttgagccgcgctgaatggaccccgcaggatttggatttgatggaaattaatgaagcctttgctgcccaagctttggccgtccatcagcaaatgggttgggatacctctaaagtcaatgttaatggtggcgctattgccattggccatcctattggcgccagcggttgtcgtattttggttaccttgttgcatgaaatgaaacgtcgcgatgccaaaaaaggcttggctagcttgtgcattggtggcggtatgggtgtcgctttggccgttgaacgcaaataa
Source
This gene is originally from Ralstonia eutropha.GenScript synthesized the gene and optimized its codon for us.