Difference between revisions of "Part:BBa K3096025"
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<partinfo>BBa_K3096025 short</partinfo> | <partinfo>BBa_K3096025 short</partinfo> | ||
Used to measure the expression of MntR and give a mRFP read out. If pMnt is present, the system should be repressed and no mRFP signal should be detectable. | Used to measure the expression of MntR and give a mRFP read out. If pMnt is present, the system should be repressed and no mRFP signal should be detectable. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
The pMnt is a promoter originating from the Salmonella bacteriophage P22. | The pMnt is a promoter originating from the Salmonella bacteriophage P22. | ||
| − | + | This promoter is regualted by MntR. If MntR binds to the promoter transcription of downstream genes is inhibited. | |
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| + | ===Sequence and Features=== | ||
<partinfo>BBa_K3096025 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3096025 SequenceAndFeatures</partinfo> | ||
Revision as of 14:47, 15 October 2019
pMnt-RFP reporter for Mnt expression
Used to measure the expression of MntR and give a mRFP read out. If pMnt is present, the system should be repressed and no mRFP signal should be detectable.
Usage and Biology
The pMnt is a promoter originating from the Salmonella bacteriophage P22.
This promoter is regualted by MntR. If MntR binds to the promoter transcription of downstream genes is inhibited.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NotI site found at 781 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1555
Illegal AgeI site found at 1667 - 1000COMPATIBLE WITH RFC[1000]