Difference between revisions of "Part:BBa K2984019"
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==Usage and Biology== | ==Usage and Biology== | ||
| − | This construct is designed for the expression of the yellow fluorescent protein mVenus in <i>C. reinhardtii</i>. | + | This construct is designed for the expression of the yellow fluorescent protein mVenus in <i>C. reinhardtii</i>. It is composed of the PsaD promoter, which stems from the photosystem one complex, the mVenus coding sequence and the terminator Rbcs2, taken from the Rubisco gene. This construct will make <i>C. reinhardtii</i> glow under a fluorescence microscope. |
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| + | With this construct we focused on the characterization of the light induction of PsaD. | ||
==Characterization== | ==Characterization== | ||
| + | |||
| + | The first qualitative characterization we made was to check the fluorescence of <i>C. reinhardtii</i> that had been transformed with this construct under the microscope. | ||
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Revision as of 14:42, 15 October 2019
L1c-Psad-YFP-RbcS2, Expression of YFP through light-inducible promoter
This Level 1 Construct is designed for comparison of locus dependent expression through fluorescence intensity measuerments. The Construct consist of the Psad promoter an coding sequence for the yellow fluorescent protein and the Rbcs2 terminator. The promoter is inducible by light.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1046
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1046
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1046
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1046
Illegal NgoMIV site found at 1523 - 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
This construct is designed for the expression of the yellow fluorescent protein mVenus in C. reinhardtii. It is composed of the PsaD promoter, which stems from the photosystem one complex, the mVenus coding sequence and the terminator Rbcs2, taken from the Rubisco gene. This construct will make C. reinhardtii glow under a fluorescence microscope.
With this construct we focused on the characterization of the light induction of PsaD.
Characterization
The first qualitative characterization we made was to check the fluorescence of C. reinhardtii that had been transformed with this construct under the microscope.