Difference between revisions of "Part:BBa K2999004"

Revision as of 14:40, 15 October 2019

PA2069 Promoter

This part is a promoter which is regulated by the quorum sensing system in Pseudomonas aeruginosa, and the signal molecule which regulates the promoter is N-(butyry1)-1-homoserine lactone (HSL).

Usage and Biology

In Pseudomonas aeruginosa PAO1, HSL signal molecules need to form a complex with rhlr to start the downstream gene transcription. We knock out genes related to HSL signal molecules and PQS signal molecules, and find that when HSL signal is missing, PA2069 loses its β-Galactosidase activity, that is, PA2069 is the promoter responding to HSL.

Figure 1.Determination of β - galactosidase activity of pa2069 promoter

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 365
Illegal XhoI site found at 698
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 96
Illegal NgoMIV site found at 271
Illegal NgoMIV site found at 297
Illegal NgoMIV site found at 482
Illegal NgoMIV site found at 602
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 989

@@ Line 5: / Line 5: @@
 This part is a promoter which is regulated by the quorum sensing system in Pseudomonas aeruginosa, and the signal molecule which regulates the promoter is N-(butyry1)-1-homoserine lactone (HSL).
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+In Pseudomonas aeruginosa PAO1, HSL signal molecules need to form a complex with rhlr to start the downstream gene transcription. We knock out genes related to HSL signal molecules and PQS signal molecules, and find that when HSL signal is missing, PA2069 loses its β-Galactosidase activity, that is, PA2069 is the promoter responding to HSL.
+[[File:T--YAU-China--iGEM2019-BBa -6.png|500px|thumb|none|Figure 1.Determination of β - galactosidase activity of pa2069 promoter ]]
 <!-- -->