Difference between revisions of "Part:BBa K3096025"

 
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<partinfo>BBa_K3096025 short</partinfo>
 
<partinfo>BBa_K3096025 short</partinfo>
  
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Used to measure the expression of MntR, thus the sensing of GlcNAc. If GlcNAc is sensed, the reporter should lose its ability to express RFP.
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Used to measure the expression of MntR and give a mRFP read out. If pMnt is present, the system should be repressed and no mRFP signal should be detectable.
  
 
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his promoter is regualted by MntR. If MntR binds to the promoter transcription of downstream genes is inhibited.
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===Usage and Biology===
 
===Usage and Biology===
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The pMnt is a promoter originating from the Salmonella bacteriophage P22.
  
 
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Revision as of 14:39, 15 October 2019


pMnt-RFP reporter for Mnt expression

Used to measure the expression of MntR and give a mRFP read out. If pMnt is present, the system should be repressed and no mRFP signal should be detectable.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 781
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1555
    Illegal AgeI site found at 1667
  • 1000
    COMPATIBLE WITH RFC[1000]