Difference between revisions of "Part:BBa K3096005"
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Const. FadR generator (BBa_K861052 contains promoter, RBS, FadR) - FadR promoter (BBa_K861060)- RBS (BBa_K3096000). FadR binds DNA and represses gene expression for beta-oxidation in the absence of long chain Acyl-CoA | Const. FadR generator (BBa_K861052 contains promoter, RBS, FadR) - FadR promoter (BBa_K861060)- RBS (BBa_K3096000). FadR binds DNA and represses gene expression for beta-oxidation in the absence of long chain Acyl-CoA | ||
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+ | ===Background=== | ||
+ | The intake of a fatty meal increases the fatty acid availability within the body and therefore their metabolite Acyl-CoA will increase. Long chain Acyl-CoA will bind constitutively expressed FadR and hence inhibits its activity (Feng et al. 2012). FadR is the repressor of the promoter pFad | ||
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+ | ===Biology and Usage=== | ||
+ | The FadR expression system of E.Coli senses Acyl-CoA and activates fatty acid transport genes in vivo (Feng et al. 2012). | ||
+ | ===References=== | ||
+ | Feng Y, Cronan JE. Crosstalk of Escherichia coli FadR with global regulators in expression of fatty acid transport genes. PLoS One. (2012);7(9):e46275. doi:10.1371/journal.pone.0046275 | ||
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Revision as of 14:24, 15 October 2019
Constitutive FadR expression and regulated promoter
Const. FadR generator (BBa_K861052 contains promoter, RBS, FadR) - FadR promoter (BBa_K861060)- RBS (BBa_K3096000). FadR binds DNA and represses gene expression for beta-oxidation in the absence of long chain Acyl-CoA
Background
The intake of a fatty meal increases the fatty acid availability within the body and therefore their metabolite Acyl-CoA will increase. Long chain Acyl-CoA will bind constitutively expressed FadR and hence inhibits its activity (Feng et al. 2012). FadR is the repressor of the promoter pFad
Biology and Usage
The FadR expression system of E.Coli senses Acyl-CoA and activates fatty acid transport genes in vivo (Feng et al. 2012).
References
Feng Y, Cronan JE. Crosstalk of Escherichia coli FadR with global regulators in expression of fatty acid transport genes. PLoS One. (2012);7(9):e46275. doi:10.1371/journal.pone.0046275
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 902
Illegal NheI site found at 925 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]