Difference between revisions of "Part:BBa K3195004:Design"
(→Design Notes) |
(→Source) |
||
| Line 10: | Line 10: | ||
===Source=== | ===Source=== | ||
| − | + | subtilisin-like protease gene from Branchiostoma belcheri tsingtauense | |
| − | + | ||
===References=== | ===References=== | ||
Revision as of 13:14, 15 October 2019
Trx-His-EK tagged subtilisin-like protease
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 447
Illegal XhoI site found at 1645 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 889
Illegal AgeI site found at 1120 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 967
Design Notes
It is the coding sequence of subtilisin-like protease found in Branchiostoma. We add 6 his tag at its tail, besides we optimized its condons for better prokaryotic expression.
Source
subtilisin-like protease gene from Branchiostoma belcheri tsingtauense