Difference between revisions of "Part:BBa K3221200"
Line 4: Line 4:
  
 
GAL system is a galactose-mediated induction system in yeast. To be specific, GAL1 promoter can induce downstream gene expression under the control of GAL4 protein. What’s more, in order to simplify the verification of GAL1 promoter function, we chose the yeGFP as a reporter instead of Cns1 and Cns2 in our project.  
 
GAL system is a galactose-mediated induction system in yeast. To be specific, GAL1 promoter can induce downstream gene expression under the control of GAL4 protein. What’s more, in order to simplify the verification of GAL1 promoter function, we chose the yeGFP as a reporter instead of Cns1 and Cns2 in our project.  
 +
 +
[[File: T--SCU-China--scu-pGAldelay2019.png|thumb|left|'''Fig.1''' A1 and B1: WT in YPG；A2 and B2 : Transformed in YPD；A3, A4, B3 and B4: Transformed in YPG. Visible Fluorescence in B3 and B4.]]
  
  

Revision as of 12:21, 15 October 2019


Galactose-mediated induction system, yeGFP as a reporter

GAL system is a galactose-mediated induction system in yeast. To be specific, GAL1 promoter can induce downstream gene expression under the control of GAL4 protein. What’s more, in order to simplify the verification of GAL1 promoter function, we chose the yeGFP as a reporter instead of Cns1 and Cns2 in our project.

Fig.1 A1 and B1: WT in YPG；A2 and B2 : Transformed in YPD；A3, A4, B3 and B4: Transformed in YPG. Visible Fluorescence in B3 and B4.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1199