Difference between revisions of "Part:BBa K3187026"

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−	<td>coat protein with LPETGG in pET24</td>	+	<td>mCherry</td>
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Revision as of 12:03, 15 October 2019

mCherry Fluorescence Protein

name	mCherry
base pairs	705
molecular weight	26.6 kDa
origin	synthetic, derived from Discosoma sp.
properties	Red fluorescent, Ex λ: 587nm, Em λ: 610 nm

mCherry is a red fluorescent protein, which is derived from Discosoma sp.. It absorbs light with a wavelength of 587 nm and emits light with a wavelength of 610 nm. mCherry can be modified N- and C-terminal without a deficit in fluorescent.^[1]

References

1. ↑ Nathan Shaner, Robert Campbell, Paul Steinbach, Ben Giepmans, Amy Palmer and Roger Tsien, Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein, Nature Biotechnology, 2004, 22: 1567-1572 [1]

Sequence and Features




Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]