Difference between revisions of "Part:BBa K1033910"

Revision as of 11:14, 15 October 2019

fwYellow, yellow chromoprotein

This chromoprotein (also known as FezziwigYFP) naturally exhibits strong color when expressed. Compared to many other chromoproteins, such as amilCP (BBa_K592009), amilGFP (BBa_K592010), spisPink (BBa_K1033932), asPink (BBa_K1033933) and aeBlue (BBa_K864401), the color development is slower. The color is readily observed in both LB or on agar plates after 24-64 hours of incubation.

Usage and Biology

This part is useful as a reporter.

iGEM13_Uppsala: Expression of chromoproteins. The images above show E coli constitutively expressing meffRed BBa_K1033922, aeBlue BBa_K1033929, tsPurple BBa_K1033905, asPink (asCP) BBa_K1033927, fwYellow BBa_K1033910, meffBlue BBa_K1033902, meffRed BBa_K1033922 and scOrange BBa_K1033913.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 109
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 486

Characterization: NWU-China 2019

The NWU-China team overexpressed fwYellow protein in E. coli and extracted and purified. The maximum absorbance was measured in a 96-well plate using a microplate reader (Synergy 2).

Figure 1. fwYellow absorbance spectrum

At the same time, fwYellow can emit strong fluorescence under blue light excitation.

File:T--NWU-China--Characterization-yellow.jpg.png

Figure 1. under blue light excitation, The left one is fwYellow

@@ Line 29: / Line 29: @@
 [[File:T--NWU-China--characterization-BBa_K1033910%28fwyellow%29.png|900px|thumb|none|alt=fwYellow spectum.|Figure 1. fwYellow absorbance spectrum]]
+<br>
+At the same time, fwYellow can emit strong fluorescence under blue light excitation.
+[[File:T--NWU-China--Characterization-yellow.jpg.png|900px|thumb|none|alt=fluorescence of fwYellow.|Figure 1. under blue light excitation, The left one is fwYellow]]
 ;