Difference between revisions of "Part:BBa K2999000"

Revision as of 10:19, 15 October 2019

RBS+PelA

This part is mainly used to encode the extracellular polysaccharide hydrolase PelA of Pseudomonas aeruginosa PAO1 biofilm and its hydrolysis. The first 100 bp of the sequence is its own RBS region.

Usage and Biology

Please click:BBa_K2999003

YAU-China expressed the biofilm-damaged system in 2019, using extracts from each part of the system for experiments. After overnight induction of the control strain, two strains expressing single enzyme and two strains co-expressing two enzymes, four strains were collected by centrifugation and suspended in PBS buffer of equal volume. The supernatant was absorbed and added into the biofilm of Pseudomonas aeruginosa cultured on the pre-PVC plate. After incubation for a period of time, the PVC plate was cleaned, stained with crystal violet for 10 minutes, and the remaining biomass was treated with anhydrous ethanol. The coatings were washed out and OD570 was detected by enzyme labeling.

Figure 1. Effects of PelA and PslG on Biofilm

Figure 2. Crystal violet staining of biofilm treated with extracts from control group and three groups

Sequence and Features