Difference between revisions of "Part:BBa K3239006"

Revision as of 10:05, 15 October 2019

yEGFP3

A yeast- and FACS-optimized GFP variant

Usage and Biology

This yEGFP3 reporter gene is an EGFP variant optimized for yeast and FACS cell sorting.

Functional Parameters

yEGFP3 is reported to have a half-life of about 7h in Saccharomyces cerevisiae (Mateus et al., 2000), the budding yeast to which Pichia pastoris is very similar. A single-base mutation is introduced for compatibility with Type IIS. This single mutation does not affect the protein sequence.

Reference

Protein: https://www.ncbi.nlm.nih.gov/protein/82569482

Source: https://www.ncbi.nlm.nih.gov/nuccore/82569479

Mateus, C., & Avery, S. V. (2000). Destabilized green fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry. Yeast, 16(14), 1313–1323. doi: 10.1002/1097-0061(200010)16:14<1313::aid-yea626>3.0.co;2-o

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 10: / Line 10: @@
 ===Functional Parameters===
 yEGFP3 is reported to have a half-life of about 7h in Saccharomyces cerevisiae (Mateus et al., 2000), the budding yeast to which Pichia pastoris is very similar.
+A single-base mutation is introduced for compatibility with Type IIS. This single mutation does not affect the protein sequence.
 ===Reference===