Difference between revisions of "Part:BBa K3239002"
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===Usage and Biology=== | ===Usage and Biology=== | ||
The MIT1 promoter is homogenous to the P. pastoris GS115 strain. It is upregulated by the Prm1 protein, a transcription factor homogenous to the P. pastoris GS115 strain. | The MIT1 promoter is homogenous to the P. pastoris GS115 strain. It is upregulated by the Prm1 protein, a transcription factor homogenous to the P. pastoris GS115 strain. | ||
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+ | ===Functional Parameters=== | ||
+ | A point mutation is introduced for Type IIS compatibility. This might slightly affect part functionality. | ||
===Reference=== | ===Reference=== | ||
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<partinfo>BBa_K3239002 parameters</partinfo> | <partinfo>BBa_K3239002 parameters</partinfo> | ||
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Revision as of 10:04, 15 October 2019
Promoter for the Mit1 transcription factor in the P.pastoris GS115 strain
Promoter for the MIT1 gene in the P. pastoris GS115 strain. Upregulated by Prm1.
Usage and Biology
The MIT1 promoter is homogenous to the P. pastoris GS115 strain. It is upregulated by the Prm1 protein, a transcription factor homogenous to the P. pastoris GS115 strain.
Functional Parameters
A point mutation is introduced for Type IIS compatibility. This might slightly affect part functionality.
Reference
Liang, S., Wang, B., Pan, L., Ye, Y., He, M., Han, S., … Lin, Y. (2012). Comprehensive structural annotation of Pichia pastoris transcriptome and the response to various carbon sources using deep paired-end RNA sequencing. BMC Genomics, 13(1). https://doi.org/10.1186/1471-2164-13-738
Shi, L., Wang, J., Wang, X., Zhang, Y., Song, Z., Cai, M., & Zhou, X. (2019). Transcriptome and metabolome analyses reveal global behaviour of a genetically engineered methanol-independent Pichia pastoris strain. Process Biochemistry, 76(August 2018), 46–54. https://doi.org/10.1016/j.procbio.2018.10.014
Wang, X., Wang, Q., Wang, J., Zhou, M., Shi, L., Zhou, X., … Shen, W. (2016). Mit1 Transcription Factor Mediates Methanol Signaling and Regulates the Alcohol Oxidase 1 ( AOX1 ) Promoter in Pichia pastoris. Journal of Biological Chemistry, 291(12), 6245–6261. https://doi.org/10.1074/jbc.m115.692053
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 793
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 793
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 793
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 793
- 1000COMPATIBLE WITH RFC[1000]