Difference between revisions of "Part:BBa K3239006"

Revision as of 09:56, 15 October 2019

yEGFP3

A yeast- and FACS-optimized GFP variant

Usage and Biology

This yEGFP3 reporter gene is an EGFP variant optimized for yeast and FACS cell sorting.

Functional Parameters

yEGFP3 is reported to have a half-life of about 7h in Saccharomyces cerevisiae (Mateus et al., 2000), the budding yeast to which Pichia pastoris is very similar.

Reference

Protein: https://www.ncbi.nlm.nih.gov/protein/82569482 Source: https://www.ncbi.nlm.nih.gov/nuccore/82569479 Mateus, C., & Avery, S. V. (2000). Destabilized green fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry. Yeast, 16(14), 1313–1323. doi: 10.1002/1097-0061(200010)16:14<1313::aid-yea626>3.0.co;2-o

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3239006 short</partinfo>
-reporter EGFP variant optimized for yeast for FACS
+A yeast- and FACS-optimized GFP variant
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+This yEGFP3 reporter gene is an EGFP variant optimized for yeast and FACS cell sorting.
+===Functional Parameters===
+yEGFP3 is reported to have a half-life of about 7h in Saccharomyces cerevisiae (Mateus et al., 2000), the budding yeast to which Pichia pastoris is very similar.
+===Reference===
+Protein: https://www.ncbi.nlm.nih.gov/protein/82569482
+Source: https://www.ncbi.nlm.nih.gov/nuccore/82569479
+Mateus, C., & Avery, S. V. (2000). Destabilized green fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry. Yeast, 16(14), 1313–1323. doi: 10.1002/1097-0061(200010)16:14<1313::aid-yea626>3.0.co;2-o
+<!-- Add more about the biology of this part here
 <!-- -->
@@ Line 14: / Line 25: @@
 <!-- Uncomment this to enable Functional Parameter display
-===Functional Parameters===
 <partinfo>BBa_K3239006 parameters</partinfo>
 <!-- -->