Difference between revisions of "Part:BBa K3044024"
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Cas9-enzymes are DNA specific endonucleases, which perform double stranded breaks in the target DNA. To identify the target, the protein uses an RNA molecule referred to as a single guide RNA (sgRNA) which forms a complex with the Cas9 protein and functions as its guide towards the target. [https://www.ncbi.nlm.nih.gov/pubmed/24906146] | Cas9-enzymes are DNA specific endonucleases, which perform double stranded breaks in the target DNA. To identify the target, the protein uses an RNA molecule referred to as a single guide RNA (sgRNA) which forms a complex with the Cas9 protein and functions as its guide towards the target. [https://www.ncbi.nlm.nih.gov/pubmed/24906146] | ||
| − | This Cas9 is | + | This Cas9 is activated by making A10D and A840H mutations of <partinfo>BBa_K3044008</partinfo> to activate the catalytic domains of the protein. |
Latest revision as of 08:56, 15 October 2019
Codon optimized Cas9 for expression in E. coli
Cas9 is a protein with endonuclease activity and induces a double stranded break in DNA.
Cas9 is the active protein in the CRISPR/Cas9 system II. It has its origin in Streptococcus Pyogenes and can be used as a gene editing tool for specific target genes.
Cas9-enzymes are DNA specific endonucleases, which perform double stranded breaks in the target DNA. To identify the target, the protein uses an RNA molecule referred to as a single guide RNA (sgRNA) which forms a complex with the Cas9 protein and functions as its guide towards the target. [1]
This Cas9 is activated by making A10D and A840H mutations of BBa_K3044008 to activate the catalytic domains of the protein.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 251
Illegal BglII site found at 1325 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]