Difference between revisions of "Part:BBa K2936007"

 
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An alpha peptide chain encoded by a gene LacZ
 
An alpha peptide chain encoded by a gene LacZ
  
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===Usage and Biology===
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===<h1>Usage and Biology</h1>===
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<p>
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  The LacZ alpha gene is one of the structural genes present on the Lac Operon in Escherichia coli bacteria . When transcribed by RNA polymerase , the LacZ alpha gene codes for β-galactosidase , an enzyme responsible for the hydrolysis of β-galactosides (e.g. 2-Nitrophenyl β-D-galactopyranoside (ONPG) )
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</p>
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===<h1>Characterize</h1>===
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<p>
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LacZ alpha gene encodes the β-galactosidase, which could react with 2-Nitrophenyl β-D-galactopyranoside (ONPG) to generate orange color.
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<br>Mechanism:
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  <img src="https://2019.igem.org/File:T--ZJUT-China--igem-part07jili.png">
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</p>
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===<h1>Experimental Results</h1>===
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<p>Form 1 The absorbance of different reaction solution at the wavelength of350 nm</p>
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  <img src="https://2019.igem.org/File:T--ZJUT-China--igem-part07form.png">
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  <img src="https://2019.igem.org/File:T--ZJUT-China--igem-part07reaction.jpg">
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<p>Figure 7.1 The ration of ONPG is 1:2,1:4,1:8,1:10,1:15,1:20 from the left to right.</p>
  
 
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Revision as of 06:25, 15 October 2019


LacZ alpha

An alpha peptide chain encoded by a gene LacZ


Usage and Biology

The LacZ alpha gene is one of the structural genes present on the Lac Operon in Escherichia coli bacteria . When transcribed by RNA polymerase , the LacZ alpha gene codes for β-galactosidase , an enzyme responsible for the hydrolysis of β-galactosides (e.g. 2-Nitrophenyl β-D-galactopyranoside (ONPG) )

Characterize

LacZ alpha gene encodes the β-galactosidase, which could react with 2-Nitrophenyl β-D-galactopyranoside (ONPG) to generate orange color.
Mechanism:

Experimental Results

Form 1 The absorbance of different reaction solution at the wavelength of350 nm

Figure 7.1 The ration of ONPG is 1:2,1:4,1:8,1:10,1:15,1:20 from the left to right.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]