Difference between revisions of "Part:BBa K3031009"

 
 
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<partinfo>BBa_K3031009 short</partinfo>
 
<partinfo>BBa_K3031009 short</partinfo>
  
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BBa_K1830001 BBa_K3031004 BBa_K3031000 BBa_J18921 BBa_K3031002 BBa_B0015
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This part contains a rbs (part BBa_) sequence followed by the sequence for Signalling peptide Lp_2145 (part BBa_K3031003) which is flanked by two restriction sites (NdeI and Spel) creating a signalling peptide cassette which can be easily exchanged with another to test the efficiency of exporting of heterlogous proteins in lactobacillus spp.
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The protein to be exported or secreted gene of interest can be fused to the end of this sequence. Another signalling peptide can easily replace the SP by digestion and ligation for later experiments into SP efficiency.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 00:09, 15 October 2019


Signalling Peptide Lp_2145 + ORF81 + Cwa

This part contains a rbs (part BBa_) sequence followed by the sequence for Signalling peptide Lp_2145 (part BBa_K3031003) which is flanked by two restriction sites (NdeI and Spel) creating a signalling peptide cassette which can be easily exchanged with another to test the efficiency of exporting of heterlogous proteins in lactobacillus spp.

The protein to be exported or secreted gene of interest can be fused to the end of this sequence. Another signalling peptide can easily replace the SP by digestion and ligation for later experiments into SP efficiency.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 293
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 716
    Illegal AgeI site found at 615
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 836
    Illegal SapI site found at 805