Difference between revisions of "Part:BBa K3183000"

Revision as of 20:20, 14 October 2019

Erythromycin Constitutive Promoter

P-erm is a constitutive promoter which can be used in Lactobacillus reuteri 10023C, and may have uses in other Lactobacillus species.

The promoter is derived from the erythromycin ribosomal methylase (ermB) promoter from the broad-host range plasmid pAMβ1 isolated from Enterococcus faecalis (Swinfield et al., 1990). It was characterised in six strains of Lactobacillus reuteri and Lactococcus lactis spp. cremoris MG1363 by Lizier et al, 2010.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Characterisation

This part was characterised in the composite part BBa_K3183101. Data for this part is shown below.

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3183000 short</partinfo>
-<html
+<html>
+<br>
+<br>
+P-erm is a constitutive promoter which can be used in <i>Lactobacillus reuteri</i> 10023C, and may have uses in other <i>Lactobacillus</i> species.
+<br>
 <br>
 The promoter is derived from the erythromycin ribosomal methylase (ermB) promoter from the broad-host range plasmid pAM&#946;1 isolated from Enterococcus faecalis (Swinfield et al., 1990). It was characterised in six strains of Lactobacillus reuteri and Lactococcus lactis spp. cremoris MG1363 by <html><a href="https://academic.oup.com/femsle/article/308/1/8/513999">Lizier et al, 2010.</a>
 <br>
 <br>
-It is a constitutive promoter which can be used in <i>Lactobacillus reuteri</i> 10023C.
 <br>
 </html>