Difference between revisions of "Part:BBa K3096007"
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| − | + | In the type I CISPR/Cas system the crRNA guides the surveillance complex Cascade ABCDE to | |
| + | complementary DNA sequence. The DNA is unwound, generating the so-called R-loop. The Cas3 helicase-nuclease protein attaches to this R-loop and unidirectionally degrades the DNA. Hereby, the large Cas3/Cascade complex pulls the substrate DNA towards itself, introducing single strand breaks. | ||
Revision as of 19:33, 14 October 2019
In the type I CISPR/Cas system the crRNA guides the surveillance complex Cascade ABCDE to complementary DNA sequence. The DNA is unwound, generating the so-called R-loop. The Cas3 helicase-nuclease protein attaches to this R-loop and unidirectionally degrades the DNA. Hereby, the large Cas3/Cascade complex pulls the substrate DNA towards itself, introducing single strand breaks.