Difference between revisions of "Part:BBa K143031:Design"

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<partinfo>BBa_K143031 short</partinfo>
 
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<partinfo>BBa_K143031 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K143031 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
 
The sequence of ''B. subtilis'' integration vector pDR111 was searched for the spectinomycin resistance gene and the Biobrick standard applied to the gene sequence upon PCR cloning
 
The sequence of ''B. subtilis'' integration vector pDR111 was searched for the spectinomycin resistance gene and the Biobrick standard applied to the gene sequence upon PCR cloning
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Revision as of 13:48, 18 September 2008

Aad9 Spectinomycin Resistance Gene



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 488


Design Notes

The sequence of B. subtilis integration vector pDR111 was searched for the spectinomycin resistance gene and the Biobrick standard applied to the gene sequence upon PCR cloning


Source

Aad9 was PCR cloned from the B. subtilis integration vector pDR111 using Pfu DNA polymerase