Difference between revisions of "Part:BBa K3015006:Design"
| Line 8: | Line 8: | ||
===Design Notes=== | ===Design Notes=== | ||
The T7-polymerase will start transcription from a T7-promotor | The T7-polymerase will start transcription from a T7-promotor | ||
| + | |||
| + | We Mutated the following basepairs to delete BbsI/BpiI recognition sites | ||
| + | - S202 changed from TCT to TCC (bp 604-606) | ||
| + | - E207 changed from GAA to GAG (bp 619-621) | ||
| + | - E309 changed from GAA to GAG (bp 925-927) | ||
| + | - E365 changed from GAA to GAG (bp 1093-1095) | ||
| + | - K714 changed from AAG to AAA (bp 2140-21429 | ||
Revision as of 16:52, 14 October 2019
T7 RNA Polymerase (No BbsI/BpiI)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The T7-polymerase will start transcription from a T7-promotor
We Mutated the following basepairs to delete BbsI/BpiI recognition sites - S202 changed from TCT to TCC (bp 604-606) - E207 changed from GAA to GAG (bp 619-621) - E309 changed from GAA to GAG (bp 925-927) - E365 changed from GAA to GAG (bp 1093-1095) - K714 changed from AAG to AAA (bp 2140-21429
Source
T7 polymerase from BBa_K145001