Difference between revisions of "Part:BBa K3286041:Design"

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===Design Notes===
 
===Design Notes===
 
Due to the fact that repeat regions are difficult to chemically synthesize, hard to clone and in general generate high number of errors, we were looking for a simpel and modular system for Cpf1 spacer-repeat production.  
 
Due to the fact that repeat regions are difficult to chemically synthesize, hard to clone and in general generate high number of errors, we were looking for a simpel and modular system for Cpf1 spacer-repeat production.  
−
Another system like our system was already present in the iGEM repository, but this part contained BsaI sites and was not compatible with our backbone.
+
Another system like our system was already present in the iGEM repository (BBa_K2003001), but this part contained BsaI sites and was not compatible with our backbone.
  
  

Revision as of 16:37, 14 October 2019


F. novicida Cpf1 (Cas12a) CRISPR array


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Due to the fact that repeat regions are difficult to chemically synthesize, hard to clone and in general generate high number of errors, we were looking for a simpel and modular system for Cpf1 spacer-repeat production. Another system like our system was already present in the iGEM repository (BBa_K2003001), but this part contained BsaI sites and was not compatible with our backbone.


Source

Chemically synthesized F. novicida CRISPR array

References

Zetsche, B., Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., … Zhang, F. (2015). Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell, 163(3), 759–771. https://doi.org/10.1016/j.cell.2015.09.038