Difference between revisions of "Part:BBa K143036:Design"

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Revision as of 13:10, 18 September 2008

Xylose operon regulatory protein



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The wild-type XylR protein was identified using the complete genome sequene of B. subtilis #1 and PCR primers designed from this sequence. Biobrick prefix and suffix standards were added to the XylR sequence and the gene cloned by PCR with Pfu DNA polymerase.

Source

The XylR protein was PCR cloned from the B. subtilis genome using Pfu DNA polymerase

References

<biblio>

  1. 1 pmid=9384377

</biblio>