Difference between revisions of "Part:BBa K1807009"
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<p>[[File:T--Nanjing-China--bronze1.png|800px|thumb|center|Figure 1）supernatant Pi concentration in SMW]] </p>
 
<p>[[File:T--Nanjing-China--bronze1.png|800px|thumb|center|Figure 1）supernatant Pi concentration in SMW]] </p>
 
<p>[[File:T--Nanjing-China--bronze2.png|800px|thumb|center|Figure 2）optical density of Ecoil in SMW]] </p>
 
<p>[[File:T--Nanjing-China--bronze2.png|800px|thumb|center|Figure 2）optical density of Ecoil in SMW]] </p>
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<p>Reference:
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Wang X , Wang X , Hui K , et al. Highly Effective Polyphosphate Synthesis, Phosphate Removal and Concentration Using Engineered Environmental Bacteria Based on a Simple Solo Medium-copy Plasmid Strategy[J]. Environmental Science & Technology, 2017:acs.est.7b04532.</p>

Revision as of 15:42, 14 October 2019


Polyphosphate kinase enzyme from Escherichia coli

Coding sequence for the Polyphosphate enzyme (PPK) from Escherichia coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 317
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1671
  • 1000
    COMPATIBLE WITH RFC[1000]


iGEM2019_Nanjing China Experiment

This year our team develops a simple solo medium-copy plasmid-based polyphosphate kinase (PPK1) overexpression strategy for achieving maximum intracellular polyphosphate accumulation, so the data can provide some reference to this part.

We test supernatant Pi concentration and optical density of Ecoil(DH5a) and DH5a-MDPP in Synthetic municipal wastewater(SMW).

Ps: SMW means Synthetic municipal wastewater DH5a-MDPP means solo medium-copy DH5a ppk in DH5a

Figure 1）supernatant Pi concentration in SMW

Figure 2）optical density of Ecoil in SMW

Reference: Wang X , Wang X , Hui K , et al. Highly Effective Polyphosphate Synthesis, Phosphate Removal and Concentration Using Engineered Environmental Bacteria Based on a Simple Solo Medium-copy Plasmid Strategy[J]. Environmental Science & Technology, 2017:acs.est.7b04532.