Difference between revisions of "Part:BBa J100034"
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The primer sequences for individual promoters used for our synthetic biology project are as follows: | The primer sequences for individual promoters used for our synthetic biology project are as follows: | ||
| − | groe-up CGCGGATCCCCAATACTGTTTTCTCAAATGGTATGTA | + | |
| + | groe-up CGCGGATCCCCAATACTGTTTTCTCAAATGGTATGTA BamHI | ||
| + | |||
groe-down CGGGGTACCTGAAATAACCTCCTCAATAGTATGA KpnI | groe-down CGGGGTACCTGAAATAACCTCCTCAATAGTATGA KpnI | ||
Revision as of 15:39, 14 October 2019
groE promoter
The default state of this promoter is off and can be induced by heat. It is 44 base pairs long and is located 72 bases upstream of the start site of groE. The groE gene is from E. coli.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Promoters and Their Primers
Several promoters with high expression level that are commonly used in Bacillus subtilis were screened to determine the most efficient ones for our experiments on the construction of biological parts. Three of such promoters, Groe (BBa J100034), SecA (BBa K1469002), P43 (BBa K208002), exist in the iGEM registry already.Here we only show the data of Groe(BBa_J100034).
The primer sequences for individual promoters used for our synthetic biology project are as follows:
groe-up CGCGGATCCCCAATACTGTTTTCTCAAATGGTATGTA BamHI
groe-down CGGGGTACCTGAAATAACCTCCTCAATAGTATGA KpnI