Difference between revisions of "Part:BBa K3132019"

 
 
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__NOTOC__
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<partinfo>BBa_K3132019 short</partinfo>
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Flag-tag is a segment composed of 8 amino acid residues, playing a role of labeling in protein expression and location. As a fusion expression label, flag usually does not interact with the target protein and usually does not affect the functional properties of the target protein. As it can be recognized by anti-flag antibodies, we hope fused several flag tags to IL-15 protein will enhance the ability of inducing receptor dimerization. Here we fused 8 flags to IL-15. Moreover, to determine the best amount of these peptide tags, we set different enzyme cutting sites between every 2 flag single chains, after cutting with different restriction enzymes, we got the other three types of IL-15 proteins labeled with different lengths of flags: IL-15-flag6×, IL-15-flag4×, IL-15-flag2×.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3132019 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K3132019 parameters</partinfo>
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Latest revision as of 15:19, 14 October 2019


8*Flag

Flag-tag is a segment composed of 8 amino acid residues, playing a role of labeling in protein expression and location. As a fusion expression label, flag usually does not interact with the target protein and usually does not affect the functional properties of the target protein. As it can be recognized by anti-flag antibodies, we hope fused several flag tags to IL-15 protein will enhance the ability of inducing receptor dimerization. Here we fused 8 flags to IL-15. Moreover, to determine the best amount of these peptide tags, we set different enzyme cutting sites between every 2 flag single chains, after cutting with different restriction enzymes, we got the other three types of IL-15 proteins labeled with different lengths of flags: IL-15-flag6×, IL-15-flag4×, IL-15-flag2×.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 280
    Illegal NheI site found at 583
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 595
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 73
    Illegal SapI.rc site found at 223
    Illegal SapI.rc site found at 373