Difference between revisions of "Part:BBa K3102025"

 
 
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<partinfo>BBa_K3102025 short</partinfo>
 
<partinfo>BBa_K3102025 short</partinfo>
  
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This T7-RBS-TEVp-TEV-hlyA-ter (secreted TEV) composite is engaged into the TEV protease synthetization and secretion.
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The TEVp (BBa_K3102012) and HlyA basic parts (BBa_K554002) are present in this composite. The HlyA sequence is added in C-terminal as a secretion signal peptide recognized by the bacterial secretion system. The TEV peptide signal (BBa_K3102041) is used as a recognition signal to remove the signal peptide, HlyA by the TEV protease.
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This construct is enriched with T7 promoter, RBS and terminator.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 14:41, 14 October 2019


T7-RBS-TEVp-ter

This T7-RBS-TEVp-TEV-hlyA-ter (secreted TEV) composite is engaged into the TEV protease synthetization and secretion.

The TEVp (BBa_K3102012) and HlyA basic parts (BBa_K554002) are present in this composite. The HlyA sequence is added in C-terminal as a secretion signal peptide recognized by the bacterial secretion system. The TEV peptide signal (BBa_K3102041) is used as a recognition signal to remove the signal peptide, HlyA by the TEV protease.

This construct is enriched with T7 promoter, RBS and terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 541
    Illegal NgoMIV site found at 847
    Illegal AgeI site found at 202
  • 1000
    COMPATIBLE WITH RFC[1000]