Difference between revisions of "Part:BBa K143036:Design"

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===Design Notes===
 
===Design Notes===
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The XylR protein was identified in the genome using its Genbank entry[2] and NCBI's sequence viewer and PCR primers designed from the sequence. Biobrick prefix and suffix sequences were added and the gene cloned by PCR with Pfu DNA polymerase
+
 
 +
The wild-type XylR protein was identified using the complete genome sequene of B. subtilis <cite>#1</cite> and PCR primers designed from this sequence. Biobrick prefix and suffix standards were added to the XylR sequence and the gene cloned by PCR with Pfu DNA polymerase.
  
 
===Source===
 
===Source===
  
−
The XylR protein was PCR cloned form the ''B. subtilis''  genome using Pfu DNA polymerase
+
The XylR protein was PCR cloned from the ''B. subtilis''  genome using Pfu DNA polymerase
  
 
===References===
 
===References===
 
<biblio>
 
<biblio>
−
#1 pmid=2544559
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#1 pmid=9384377
−
#2 http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene, Gene ID:939531
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</biblio>
 
</biblio>

Revision as of 11:25, 18 September 2008

Xylose operon regulatory protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The wild-type XylR protein was identified using the complete genome sequene of B. subtilis #1 and PCR primers designed from this sequence. Biobrick prefix and suffix standards were added to the XylR sequence and the gene cloned by PCR with Pfu DNA polymerase.

Source

The XylR protein was PCR cloned from the B. subtilis genome using Pfu DNA polymerase

References

<biblio>

  1. 1 pmid=9384377

</biblio>