Difference between revisions of "Part:BBa K3102032"

 
 
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<partinfo>BBa_K3102032 short</partinfo>
 
<partinfo>BBa_K3102032 short</partinfo>
  
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This T7-RBS-ZWF-ter composite part is made of the glucose-6-phosphate 1-dehydrogenase enzyme which catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone in the pentose phosphate pathway.  Due to the knock-out of PGI, there is a metabolic flux shift toward the pentose phosphate shunt using this enzyme to allow the bacteria to survive. This part is useful to increase flavin production.
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This construct is enriched with T7 promoter, RBS and terminator.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 13:21, 14 October 2019


T7-RBS-ZWF-Ter

This T7-RBS-ZWF-ter composite part is made of the glucose-6-phosphate 1-dehydrogenase enzyme which catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone in the pentose phosphate pathway. Due to the knock-out of PGI, there is a metabolic flux shift toward the pentose phosphate shunt using this enzyme to allow the bacteria to survive. This part is useful to increase flavin production.

This construct is enriched with T7 promoter, RBS and terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 810
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 810
    Illegal NotI site found at 691
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1367
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 810
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 810
    Illegal NgoMIV site found at 83
    Illegal NgoMIV site found at 800
  • 1000
    COMPATIBLE WITH RFC[1000]