Difference between revisions of "Part:BBa K3102026"
 
(2 intermediate revisions by 2 users not shown)
Line 3: Line 3:
 
<partinfo>BBa_K3102026 short</partinfo>
 
<partinfo>BBa_K3102026 short</partinfo>
  
−
This T7-LDHA-ter composite composes of LDHA part (BBa_K3102006) is used in order to secrete both CAE and EG1 proteins by E.coli.
+
This T7-RBS-LDHA-ter composite is made of LDHA part (BBa_K3102006) which is used to catalyze the conversion of pyruvate in L-Lactate with concomitant inter-conversion of NADH and NAD+.  
−
This constructs is enriched with primer, T7 promoters, RBS and terminator.
+
 
 +
This construct is enriched with T7 promoter, RBS and terminator.
  
  

Latest revision as of 13:12, 14 October 2019


T7-RBS-LDHA-Ter

This T7-RBS-LDHA-ter composite is made of LDHA part (BBa_K3102006) which is used to catalyze the conversion of pyruvate in L-Lactate with concomitant inter-conversion of NADH and NAD+.

This construct is enriched with T7 promoter, RBS and terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 489
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 219