Difference between revisions of "Part:BBa K3111032:Design"
(→Design Notes) |
(→References) |
||
(One intermediate revision by the same user not shown) | |||
Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
− | + | Sequence was optimised for E. coli expression. Two illegal PstI sites removed from 44-Q and 95-Q (CAG to CAA). 15aa targeting peptide was chosen based on work by Cassidy-Amstutz et al, 2016. | |
===Source=== | ===Source=== | ||
Line 14: | Line 14: | ||
===References=== | ===References=== | ||
+ | |||
+ | Cassidy-Amstutz C, Oltrogge L, Going CC, Lee A, Teng P, Quintanilla D, et al. Identification of a Minimal Peptide Tag for in Vivo and in Vitro Loading of Encapsulin. Biochemistry. 2016 Jun 21;55(24):3461– |
Latest revision as of 11:36, 14 October 2019
Photosensitiser miniSOG fused to T. maritima encapsulin loading peptide
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 346
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Sequence was optimised for E. coli expression. Two illegal PstI sites removed from 44-Q and 95-Q (CAG to CAA). 15aa targeting peptide was chosen based on work by Cassidy-Amstutz et al, 2016.
Source
MiniSOG was derived from Arabidopsis thaliana. T. maritima targeting peptide taken from Cassidy-Amstutz et al, 2016.
References
Cassidy-Amstutz C, Oltrogge L, Going CC, Lee A, Teng P, Quintanilla D, et al. Identification of a Minimal Peptide Tag for in Vivo and in Vitro Loading of Encapsulin. Biochemistry. 2016 Jun 21;55(24):3461–