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| − | __NOTOC__
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| − | <partinfo>BBa_K3076300 short</partinfo>
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| − | <partinfo>BBa_K3076300 SequenceAndFeatures</partinfo>
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| − | ===Source===
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| − | The genomic sequence of <i>CopA</i> gene was extracted from NCBI (ACCESSION: NC_000913 REGION: complement (508875..511379)). The kanamycin resistance gene was extracted from the sequence of pET28a plasmid sequence from addgene.org. Double terminator was extracted from iGEM part registry <html>(<a href="https://parts.igem.org/Part:BBa_B0015">BBa B0015</a>)</html> which is the most commonly used double terminator with high efficiency.
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| − | ===Design Notes===
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| − | The recombination site was chosen to be near the gene's 5' end of the coding strand to increase the chance of successful knockout.
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| − | ===References===
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