Difference between revisions of "Part:BBa K3076300:Design"
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<partinfo>BBa_K3076300 short</partinfo>
 
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<partinfo>BBa_K3076300 SequenceAndFeatures</partinfo>
 
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===Source===
 
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The genomic sequence of CopA gene was  extracted from NCBI (ACCESSION: NC_000913 REGION: complement (508875..511379)). The kanamycin resistance gene was extracted from the sequence of pET28a plasmid sequence.
 
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===Design Notes===
 
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The recombination site was chosen to be near the C terminal of the gene to increase the chance of sucessful knockout.
 
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===References===
 

Latest revision as of 11:22, 14 October 2019