Difference between revisions of "Part:BBa K3112020"
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<partinfo>BBa_K3112020 short</partinfo> | <partinfo>BBa_K3112020 short</partinfo> | ||
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+ | Hygromycin B is an aminoglycosidic antibiotic that inhibits protein synthesis by disrupting translocation and promoting mistranslation at the 80S ribosome. Because it uses a different mode of action than Gibco Geneticin, Blasticidin S, or Gibco Zeocin, it’s perfect for dual-selection experiments when used in conjunction with another selection agent.It is also widely used in yeast. | ||
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<partinfo>BBa_K3112020 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3112020 SequenceAndFeatures</partinfo> |
Latest revision as of 10:59, 14 October 2019
Hygromycin Resistance Gene (hyg or hph)
Hygromycin B is an aminoglycosidic antibiotic that inhibits protein synthesis by disrupting translocation and promoting mistranslation at the 80S ribosome. Because it uses a different mode of action than Gibco Geneticin, Blasticidin S, or Gibco Zeocin, it’s perfect for dual-selection experiments when used in conjunction with another selection agent.It is also widely used in yeast.
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 238
Illegal PstI site found at 328 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 238
Illegal PstI site found at 328 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 238
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 238
Illegal PstI site found at 328 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 238
Illegal PstI site found at 328 - 1000COMPATIBLE WITH RFC[1000]