Difference between revisions of "Part:BBa K3041001"

 
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Coding gene of suckerin-9 from the Humboldt squid <i>Dosidicus gigas</i>, codon optimized for expression in <i>E. coli</i>.
 
Coding gene of suckerin-9 from the Humboldt squid <i>Dosidicus gigas</i>, codon optimized for expression in <i>E. coli</i>.
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==Validation==
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The successfully synthesized suckerins, codon-optimized for E.coli, were PCR amplified using the standard biobrick primers. In Figure 1, the PCR products of these specific suckerins were visualised on agarose gel. Suckerin-9, shows strong bands on agarose gel.
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[[File:PCR suckerin proteins.png]]
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<small><b>Figure 1: Amplified suckerin genes 8, 9 and 12.</b>
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Polymerase chain reaction (PCR) products amplified with general prefix and suffix primers, shown by gel electrophoresis. Lane 1: 1kb ladder. PCR product at different concentrations of suckerin-8 (408 bp, lane 2 and 3), suckerin-9 (568 bp, lane 4 and 5), and suckerin-12 (696 bp, lane 6 and 7). The gel confirms the amplification of the desired suckerin proteins.</small>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 08:33, 14 October 2019


Suckerin-9


Coding gene of suckerin-9 from the Humboldt squid Dosidicus gigas, codon optimized for expression in E. coli.

Validation

The successfully synthesized suckerins, codon-optimized for E.coli, were PCR amplified using the standard biobrick primers. In Figure 1, the PCR products of these specific suckerins were visualised on agarose gel. Suckerin-9, shows strong bands on agarose gel.


PCR suckerin proteins.png


Figure 1: Amplified suckerin genes 8, 9 and 12. Polymerase chain reaction (PCR) products amplified with general prefix and suffix primers, shown by gel electrophoresis. Lane 1: 1kb ladder. PCR product at different concentrations of suckerin-8 (408 bp, lane 2 and 3), suckerin-9 (568 bp, lane 4 and 5), and suckerin-12 (696 bp, lane 6 and 7). The gel confirms the amplification of the desired suckerin proteins.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 527
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]