Difference between revisions of "Part:BBa K3140004"
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PsiH (codon optimised) is a cytochrome P450 monooxygenase, which catalyses the conversion of tryptamine to 4-hydroxytryptamine. | PsiH (codon optimised) is a cytochrome P450 monooxygenase, which catalyses the conversion of tryptamine to 4-hydroxytryptamine. | ||
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| + | *NCBI: [https://www.ncbi.nlm.nih.gov/protein/ASU62239.1 ASU62239.1] | ||
| + | *UniProt: [https://www.uniprot.org/uniprot/P0DPA6 P0DPA6] | ||
| + | *EC number: [https://enzyme.expasy.org/EC/4.1.1.105 4.1.1.105] | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
PsiH (codon optimised) is an enzyme derived from ''Psilocybe cubensis'', which is involved in the metabolic biosynthesis of psilocybin from tryptophan. The coding sequence has been codon optimised for expression in ''Escherichia coli''. It accepts tryptamine as a substrate to yield 4-hydroxytryptamine, respectively. Heterologous expression of PsiH has been achieved in ''Escherichia coli'' <add strain> in a <expression system>. | PsiH (codon optimised) is an enzyme derived from ''Psilocybe cubensis'', which is involved in the metabolic biosynthesis of psilocybin from tryptophan. The coding sequence has been codon optimised for expression in ''Escherichia coli''. It accepts tryptamine as a substrate to yield 4-hydroxytryptamine, respectively. Heterologous expression of PsiH has been achieved in ''Escherichia coli'' <add strain> in a <expression system>. | ||
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| + | [[Image:T--Sydney_Australia--PsiD_KEGG_rxn1.gif|thumb|900px|Fig. 1: Decarboxylation of L-tryptophan to tryptamine, mediated by PsiD. CO<sub>2</sub> is released as a byproduct. Source: [https://www.genome.jp/dbget-bin/www_bget?reaction+R00685 KEGG] ]] | ||
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| + | [[Image:T--Sydney_Australia--PsiD_KEGG_rxn2.gif|thumb|900px|Fig. 2: Decarboxylation of 4-hydroxy-L-tryptophan to 4-hydroxytryptamine, mediated by PsiD. CO<sub>2</sub> is released as a byproduct. Source: [https://www.genome.jp/dbget-bin/www_bget?reaction+R11932 KEGG] ]] | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 06:57, 14 October 2019
PsiH (codon optimised) - Cytochrome P450 monooxygenase from Psilocybe cubensis
PsiH (codon optimised) is a cytochrome P450 monooxygenase, which catalyses the conversion of tryptamine to 4-hydroxytryptamine.
- NCBI: ASU62239.1
- UniProt: P0DPA6
- EC number: 4.1.1.105
Usage and Biology
PsiH (codon optimised) is an enzyme derived from Psilocybe cubensis, which is involved in the metabolic biosynthesis of psilocybin from tryptophan. The coding sequence has been codon optimised for expression in Escherichia coli. It accepts tryptamine as a substrate to yield 4-hydroxytryptamine, respectively. Heterologous expression of PsiH has been achieved in Escherichia coli <add strain> in a <expression system>.
Fig. 1: Decarboxylation of L-tryptophan to tryptamine, mediated by PsiD. CO2 is released as a byproduct. Source: KEGG
Fig. 2: Decarboxylation of 4-hydroxy-L-tryptophan to 4-hydroxytryptamine, mediated by PsiD. CO2 is released as a byproduct. Source: KEGG
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]