Difference between revisions of "Part:BBa K3140000"
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PsiD is a tryptophan decarboxylase, which catalyses the conversion of L-tryptophan to tryptamine. | PsiD is a tryptophan decarboxylase, which catalyses the conversion of L-tryptophan to tryptamine. | ||
| − | [https://www.ncbi.nlm.nih.gov/protein/ASU62239.1 NCBI] | + | *[https://www.ncbi.nlm.nih.gov/protein/ASU62239.1 NCBI] |
| − | UniProt: [https://www.uniprot.org/uniprot/P0DPA6 P0DPA6] | + | *UniProt: [https://www.uniprot.org/uniprot/P0DPA6 P0DPA6] |
| − | EC number: [https://enzyme.expasy.org/EC/4.1.1.105 4.1.1.105] | + | *EC number: [https://enzyme.expasy.org/EC/4.1.1.105 4.1.1.105] |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 05:09, 14 October 2019
PsiD - Tryptophan decarboxylase from Psilocybe cubensis
PsiD is a tryptophan decarboxylase, which catalyses the conversion of L-tryptophan to tryptamine.
Usage and Biology
PsiD is a native enzyme obtained from the Psilocybe cubensis, which is involved in the metabolic biosynthesis of psilocybin from tryptophan. It accepts both L-tryptophan and 4-hydroxy-L-tryptophan as substrates, producing tryptamine and 4-hydroxytryptamine, respectively. Heterologous expression of PsiD has been achieved in Escherichia coli <add strain> in a <expression system>.
ADD:
- SDS-PAGE IMAGE - LC/MS - pUS250 and pET-28c(+)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]