Difference between revisions of "Part:BBa K3140004"

 
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<partinfo>BBa_K3140004 short</partinfo>
 
<partinfo>BBa_K3140004 short</partinfo>
  
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Codon optimised PsiH
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PsiH (codon optimised) is a cytochrome P450 monooxygenase, which catalyses the conversion of tryptamine to 4-hydroxytryptamine.
  
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===Usage and Biology===
 
===Usage and Biology===
  
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PsiH (codon optimised) is an enzyme derived from ''Psilocybe cubensis'', which is involved in the metabolic biosynthesis of psilocybin from tryptophan. The coding sequence has been codon optimised for expression in ''Escherichia coli''. It accepts tryptamine as a substrate to yield 4-hydroxytryptamine, respectively. Heterologous expression of PsiH has been achieved in ''Escherichia coli'' <add strain> in a <expression system>.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3140004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3140004 SequenceAndFeatures</partinfo>

Revision as of 01:26, 14 October 2019


PsiH (codon optimised) - Cytochrome P450 monooxygenase from Psilocybe cubensis

PsiH (codon optimised) is a cytochrome P450 monooxygenase, which catalyses the conversion of tryptamine to 4-hydroxytryptamine.

Usage and Biology

PsiH (codon optimised) is an enzyme derived from Psilocybe cubensis, which is involved in the metabolic biosynthesis of psilocybin from tryptophan. The coding sequence has been codon optimised for expression in Escherichia coli. It accepts tryptamine as a substrate to yield 4-hydroxytryptamine, respectively. Heterologous expression of PsiH has been achieved in Escherichia coli <add strain> in a <expression system>.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]