Difference between revisions of "Part:BBa K3147004:Design"
Line 1: | Line 1: | ||
+ | __NOTOC__ | ||
+ | <partinfo>BBa_K3147003 short</partinfo> | ||
+ | <partinfo>BBa_K3147003 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | ===Design note=== | ||
+ | |||
+ | The TEV cutting site used is the conventional site composed of 6 amino acids: ENLYFQS. | ||
+ | We have observed that this construction is weakly expressed. We advise future users to add a RiboJ insulator and a BCD2 element to improve its expression. |
Revision as of 19:09, 13 October 2019
mRFP1 fused to a TEV-cleavable ssrA tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 691
- 1000COMPATIBLE WITH RFC[1000]
Design note
The TEV cutting site used is the conventional site composed of 6 amino acids: ENLYFQS. We have observed that this construction is weakly expressed. We advise future users to add a RiboJ insulator and a BCD2 element to improve its expression.