Difference between revisions of "Part:BBa K3257111"

 
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<partinfo>BBa_K3257111 short</partinfo>
 
<partinfo>BBa_K3257111 short</partinfo>
  
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This part includes the gene of interest, which is ChlR in this part, and a set of necessary regulatory factor for the reverse transcription and recombination, such as the primer binding site (PBS), the R region, the U5 region, the polypurine tract (PPT) and lox sites. Together they function as a target of the R-Evolution process.
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This part includes the gene of interest, which is ChlR in this part, and a set of necessary regulatory factor for the reverse transcription and recombination, such as the primer binding site (PBS), the R region, the U5 region, the polypurine tract (PPT) and lox sites. Together they function as a target of the reverse transcription and recombination process.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:05, 13 October 2019


T7 Promoter-lox5171-R-PPT-RBS-ChlR-PBS-U5-R-loxP-T7 Terminator

This part includes the gene of interest, which is ChlR in this part, and a set of necessary regulatory factor for the reverse transcription and recombination, such as the primer binding site (PBS), the R region, the U5 region, the polypurine tract (PPT) and lox sites. Together they function as a target of the reverse transcription and recombination process.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1107
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 945
    Illegal BsaI.rc site found at 966