Difference between revisions of "Part:BBa K2922038:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Each CDS are added an RBS (BBa_B0034) behind, and contain a terminator(BBa_B0010) | + | Each CDS are added an RBS (BBa_B0034) behind, and contain a terminator(BBa_B0010) in the end of operon`s sequence |
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===Source=== | ===Source=== | ||
Latest revision as of 16:27, 13 October 2019
The colicin-N operon under T7 promoter control with a gfasPurple chromoprotein reporter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1938
Illegal NheI site found at 1961 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 598
Illegal AgeI site found at 1602 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Each CDS are added an RBS (BBa_B0034) behind, and contain a terminator(BBa_B0010) in the end of operon`s sequence
Source
This part constructs from BBa_K525998,BBa_K2922027,BBa_K2922028,BBa_K2922029,BBa_K1033917 by standard assembly.